Plasmid mapping has revolutionized molecular biology and paved the way for the biotechnology industry. On an electrophoresis gel, band b is closer to the positive end of the gel than is band a. In outbreaks, plasmid profiling is a rapid, convenient way to follow the spread. You will be tested on what domains plasmids can be found in and what the structure of a plasmid dna is. Ap biology molecular biology lab bacterial transformation worksheet 417 below is the link to the new freeresponse booklet which shows how questions and space for answers will be distributed throughout the exam booklet. Identification of bacterial plasmids based on mobility and plasmid. Recombinant dna can be used to create massive amounts of proteins for research, and has also been used to add genes to organisms or cells. We also acknowledge previous national science foundation support under grant numbers 1246120, 1525057, and 14739.
Recombinant paper plasmids cutandpaste biotechnology. Because of this effect, these substances are known as. Plasmid analysis is a useful method for epidemiologic typing of a variety of organisms. Read a short article about how restriction enzymes are used to cut bits of dna and those bits can be inserted into the genome of other organisms. If you observe the expected sizes, then your design is correct and your analysis are confirmed. Using the information from table 4,indicate where the restriction enzyme cuts are located on the plasmid. Copy number is regulated by initiation of plasmid replication 10.
Furthermore, within a single hostplasmid pair three distinct patterns of. Biology chapter 12 dna and rna worksheet answer key. What happens to the recombinant plasmid once inside a bacteria. Good activity for an advanced biology or biotechnology class. Students use a paper model to simulate this reallife process used by biotechnicians. This worksheet contains basic conceptual questions about the structure of dna. In a molecular biology laboratory, a student obtained competent e. In bacterial cells, certain autonomously replicating circular genetic elements dna are found some. Plasmid must be unable to replicate without essential replication proteins provide in. Microsoft word ap bio lab06 molecular biology worksheet wl. Cbse worksheets all classes and all subjects printable worksheets for cbse class 1.
To move the pglo plasmid dna through the cell membrane you will. Pdf several pbluescriptderived plasmids of various sizes were constructed to study the effects of multicopy plasmid size on bacterial fitness. If you spliced the dna gene into the middle of the plasmid replication site, the plasmid will not be able to replicate, and cannot be of use. Ap biology 2007 scoring guidelines question 4 continued a using the circle provided, construct a labeled diagram of the restriction map of the plasmid. Introduction to addgenes resource any newcomer who joins a molecular biology lab will undoubtedly be asked to design, modify, or construct a plasmid.
To defend when attacked by a virus, bacteria use chemical weapons that break up the dna of the virus. Their presence influences, often dramatically, the biology of the organisms that they inhabit. This quiz and worksheet combo can help you quickly determine your understanding of bacterial plasmids and the characteristics they have. If you dont see any interesting for you, use our search form on bottom v. Dna strands restriction enzymes 36 34 32 30 28 26 24 22 20 18 16 14 12 10 8 6 4 2 0 rsa i sty i both. A double digest means that you cut your plasmid with two different restriction enzymes at the same time. Inserting a plasmid into dna would allow cells to express a protein that they are lacking. Use a transformation solution containing cacl 2 ii.
If the dna is in the form of a circular dna called a plasmid, it can be copied in. This will help you to prepare for what the exam will look like. As you already have a putative map of the plasmid, you can predict which would be the expected bands. Hybridization methods are in such wide use in cell biology today that it is difficult. Direct student pairs to apply their understanding of genetics to modify plasmids. Using these data, construct a restriction map of plasmid. In this activity students will use a plasmid map to calculate the products of various restriction enzyme digests and predict the location of the dna fragments when run on a gel. The tools recombinant dna is a set of tools that allows scientists to move between genetics, biochemistry and.
Biomedical application of plasmid dna in gene therapy. Using the word choices provided in the boxes, fill in the numbered boxes with the steps of bacteria. Restriction enzymes teachers guide the biology corner. Worksheets with answers plasmid molecular cloning scribd. Episome in bacteria, a plasmid that can be inserted into the chromosome. Emerging patterns of plasmidhost coevolution that stabilize. The areas comprising the current biotechnology are bioprocess, monoclonal antibodies, and.
Cbse worksheets for class 1 contains all the important questions on maths, english, hindi, moral science, social science, general knowledge, computers and environmental studies as per cbse syllabus. This technique allows molecular biologists to quickly evaluate the success of. Empirical and molecular formula worksheet an oxide of chromium is found to have the following % composition. Your recombinant plasmid should be circular with a portion of the cell dna included. The plasmids conveniently can be cut, fused with other dna and then.
The action of these chemicals on the viral dna is shown in the diagram below. Construct a labeled map and explain 3 points maximum h h e e 40 20 30 10 h e 40 20 30 10 e h e ecori restriction point h haeiii restriction point. Although the newcomer likely knows that a plasmid is a small circular piece of dna found in bacterial cells, she may. Using this map as a guide, give the number of restriction fragments along with their associated lengths that. Jun 30, 2016 on this page you can read or download lab cloning paper plasmid worksheet ms foglia ap biology in pdf format. We biology lab cloning paper plasmid answer pdf on this page you can read or download lab cloning paper plasmid worksheet ms foglia ap biology in pdf format.
Complete the remainder of the activity sheet you began on day 1 of the lab. Manual poses a series of questions to focus and stimulate thinking about all. Students construct paper recombinant plasmids to simulate the methods genetic. Save the key about antibiotic resistance, from the bottom of the pink sheet for later use. Isolate plasmid dna and tag with a signature sequence 2. What is the first step in the cloning of a human gene. Plasmids are small, circular, double stranded, nonchromosomal dna molecules. Base your answers to questions 31 to 33 on the information below and on your howledge of biology. Plasmid definition, types and functions biology dictionary. Biology lab cloning paper plasmid answer pdf biology lab cloning paper plasmid answer pdf are you looking for ebook biology lab cloning paper plasmid answer pdf. Understand the uses of marker or reporter genes in molecular biology. Gel electrophoresis worksheet teachers pay teachers.
Partitioning is strictly controlled for low copy, but loose for high copy 8. Locate the antibiotic resistant sites on the recombinant plasmid, along mth the replication site. When the plasmid dna digested with combinations of restriction enzymes and the resulting fragments are electrophoresed, the following data is obtained. The percent composition of a compound was found to be 63. Living and nonliving things, fruits and vegetables, parts of a plant diagram, plant life cycle, life cycle of a fish, adaptation of animals, plant adaptation and life cycle of a chicken. After finishing the modification, have teams complete the modeling bacteria transformation worksheet to demonstrate their understanding of the. To help teachers produce multiple colour copies for group classes, all activity worksheets are also available as a full colour pdf download from. You will be glad to know that right now biology lab cloning paper plasmid answer pdf is available on our online library. What is normally the source plasmid for human cloning. Dna cloning with plasmid vectors molecular cell biology. This worksheet is visual and will either help drive a concept home or open their eyes to a new concept. Describe a procedure to determine which bacterial cells have been successfully transformed.
After finishing the modification, have teams complete the modeling bacteria transformation worksheet to demonstrate their understanding of the modification process. Plasmid mapping plasmids and restriction enzymes this lesson will demonstrate the principles of plasmid mapping by examining restriction digestion patterns of plasmids used in the laboratory section of the kit and determining the position of restriction enzyme recognition sites in the plasmids by use of logic. Bacteria singlecelled microbes that were one of the first types of lifeforms to evolve on earth. A virus or plasmid used in this way is known as a cloning vector, and the dna. To learn about bacterial plasmids, one of the basic tools of genetic engineering to purify plasmid dna from a bacterial culture. Since no plasmid was given to these bacteria, no growth is expected. This is great for homework, supplementary busy worksheet for a substitute teacher, or can be used as part. Ms foglia ap biology lab cloning paper plasmid answers. Following growth of the bacteria in the presence of the antibiotic, the plasmid dna can readily be isolated from the bacterial culture. Plasmid replication requires host cell functions 9. In this laboratory investigation, plasmids containing fragments of foreign dna will be used to transform escherichia coli cells, conferring antibiotic ampicillin resistance, as. Lab cloning paper plasmid worksheet ms foglia ap biology. By selecting the box below, i am agreeing to allow addgene, via its ebook service provider, hubspot, to store my email address indefinitely or until i opt out of receiving updates to the plasmids 101 ebook so that i can receive the plasmids 101 ebook and any updates to this ebook at the email address i.
Once you have selected those enzymes that cut the plasmid once, start checking these enzymes against the cell dna strand. Maybe dolly the sheep, or experiments carried out in molecular biology labs. Consult the manufacturers manual for the optimal working conditions for each enzyme. What is a plasmid and describe unique characteristics of a plasmid. In the case of dna, this is feasible for relatively short molecules such as the genomes of small viruses. A desktop resource 1st edition 2 p a g e plasmids 101. Compare the output tag sequences with the input tags 5. Bacterial transformation with green fluorescent protein pglo genetic engineering is an umbrella term that encompasses many different techniques for moving dna between different organisms. Each of the individual worksheets includes target language from the relevant lesson plan in this book. Keeping the flask with liquid agar in a water bath at 4550c can help prevent the agar from cooling too quickly. Pdf the stable maintenance of lowcopynumber plasmids in bacteria is actively driven by partition mechanisms. The plasmid should be circular with a section of human dna spliced into the circle. Experimental studies in molecular biology with transformed.
On this page you can read or download ms foglia ap biology lab cloning paper plasmid answers in pdf format. Notice how all the fragments add up to the same total. Plasmids have played, and continue to play, indispensable roles in. Scientists can typically distinguish cells that have been successfully transfected with a plasmid from those that have not by a. Empirical formula worksheet ii wiggins elementary school. Structure of plasmid types of plasmid transfer of plasmid reproduction of plasmid 1. Bacterial transformation with green fluorescent protein pglo. If you have no enzymes that will cut your plasmid only once, then reconstruct your plasmid. Do not let the agar cool so much that is begins to solidify. Transformation is the process by which an organism acquires and expresses a whole new gene. Gene cloning worksheet 20pts recombinant dna is a plasmid that has had foreign genes added to it. Making recombinant dna molecules is at the heart of the genetic engineering controversy.
Bacteria transformation activitymodeling bacteria transformation worksheet modeling bacteria transformation worksheet data and observations staple recombinant model here. In this biology learning exercise, students complete 7 short answer questions regarding dna, gene splicing, cloning, and bacterial proteins. Describe how a plasmid can be genetically modified to include a piece of foreign dna that alters the phenotype of bacterial cells transformed with the modified plasmid. Restriction enzymes are specific to a section of dna, depending on the base pairs at that section, you will analzye sections of dna and determine which restriction enzyme should be used.
The essence of cell chemistry is to isolate a particular cellular component and then analyze its chemical structure and activity. Recover bacteria from the deceased animal spleen 4. Big genetics and information transfer 3 ap central. Isolating, cloning, and sequencing dna molecular biology of the. The purpose of this technique is to introduce a foreign plasmid into bacteria, the bacteria.
This lecture note is specifically designed for medical laboratory technologists, and includes only those areas of molecular cell biology and applied genetics relevant to degreelevel understanding of modern laboratory technology. Use one or more complete sentences to answer each question. Below is a restriction map for the plasmid pgen101 total length 20 kb. Our main purpose is that these 1 rna worksheet answer key photos gallery can be a guide for you, give you more samples and also bring you what you looking for. A plasmid also called a vector in this context is a small circular dna molecule that replicates independently of the chromosomal dna in bacteria. I will include photos of the completed sequences when i get a chance, for now, just including answers to the analysis questions. Since plasmid replicon type determines inc group, the terms inc and rep type were interchangeably used to describe plasmid types. Initially, inc typing relied on introduction of a plasmid into a strain carrying another plasmid and determining whether both plasmids were stably maintained in the progeny.
1383 1632 701 185 1404 432 491 235 1242 101 356 647 976 1302 132 424 150 1133 540 1190 224 380 1026 260 848 372 1265 1114 1087 509 1421 343 136 817 236 655 841 908 772 811 1471 595